Order now, and join the other researchers who depend on GoldBio's high-quality, highly published HEPES and save 20%* when you enter code: *This offer cannot be combined with other discounts or quotes. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. Choosing the right biological buffer for your experiment can be tricky. Registration No 3,257,926) HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. Storage/Handling: Store at room temperature. The buffer choosing proces... {{ currentSelection && currentSelection.sku ? It is commonly used in cell culture media for various types of organisms and for protein studies that use it as a binding buffer and an eluent during cation exchange chromatography. It is an inherently human trait to look for patterns in objects or things that we can personalize or... How to Choose the Right Biological Buffer for My Experiment. HEPES is also used in DNA studies as a buffer solution to form precipitates of calcium phosphate and DNA and as a buffer in electroporation. Registration No 3,257,926) Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab. HEPES is one of Good's Buffers and is used in biochemistry and molecular biology. currentSelection.sku : '...' }}, {{ casNumber && casNumber.value ? The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers. A user guide for the background, experimental use and technical discussion of GoldBio biological buffers. Product Specifications Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab. Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is categorized as a "Good" buffer which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et. Tris Base is used to prepare various buffers for gel electrophoresis, column chromatography, and protein purification and can be used as an emulsifying agent. HEPES2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid. By closing this message, you consent to our, Hello, {{ user.first_name }} {{ user.last_name }}. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. currentSelection.sku : '...' }}, {{ casNumber && casNumber.value ? HEPES is one of Good's Buffers and is used in biochemistry and molecular biology. Buffers on this chart are also categorized by family. Gold Biotechnology (U.S. Our success relies on our commitment to the success of those whom we supply. casNumber.value : '...' }}, $9.99 Ground shipping (In continental US only.). Storage/Handling: Store at room temperature. A user guide for the background, experimental use and technical discussion of GoldBio biological buffers. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. It has been demonstrated that HEPES can be used to buffer mammalian cell cultures in closed systems of high and low density cultures, as well as open systems of high density culture and has shown usefulness as a buffer for in vitro fertilization and embryo culture. HEPES, Sodium Salt is a zwitterionic Good's Buffer used to prevent pH changes caused by CO2 variability and is a common alternative buffer to bicarbonate. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. Gold Biotechnology is about putting our quality products into capable hands to leads to discoveries that impact the entire world. "Good buffers" such as HEPES are attributed with the following characteristics: Registration No 3,257,926) By declining, we will only use cookies to track your authentication session and consent preferences. Registration No 3,257,927) and Goldbio (U.S. 4X PHEM buffer for preserving cell structure. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. 4X PHEM buffer for preserving cell structure, Printable Buffer pH Chart - Arranged In Order of pH. Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers. are registered trademarks of Gold Biotechnology, Inc. We use cookies and other tools on this site. Since the reported use of HEPES for colloidal gold nanostars synthesis by Xie et al., it has been used in many other seedless methods with varied modifications . By declining, we will only use cookies to track your authentication session and consent preferences. This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. In combination with the traditional Tris buffer, HEPES has also been shown to provide lower background levels and and expanded dynamic range in quantitative real-time PCR (qPCR).